منابع مشابه
Excretion patterns of glycosaminoglycans and glycoproteins in normal human urine.
Glycosaminoglycans and glycoproteins in the urine of 100 healthy, active, human subjects were examined by cellulose acetate electrophoresis and salt gradient, ion-exchange, column chromatography. The cetylpyridinium chloride (CPC) turbidity and uronic acid:creatinine ratio was also studied. Fractions were identified by electrophoretic mobility, staining reactions, susceptibility to enzyme diges...
متن کاملAnalysis of glycosaminoglycans in urine by using acridine orange fluorescence.
The fluorescence technique described here utilizes the electrostatic interaction between the polyanionic sites of glycosaminoglycans and the cationic dye Acridine Orange to analyse urinary glycosaminoglycans from patients suffering from mucopolysaccharidoses. The basis of the titration is the decrease in the fluorescence of free Acridine Orange that occurs when it is bound to polyanions. The ef...
متن کاملPregnanetriolone in paper-borne urine for neonatal screening for 21-hydroxylase deficiency: The place of urine in neonatal screening
The standard method of primary neonatal screening for congenital adrenal hyperlasia (CAH), determination of 17-hydroxyprogesterone (17OHP) in heelprick blood, is the object of recurrent controversy because of its poor diagnostic and economic efficiency. The superior ability of urinary pregnanetriolone levels to discriminate between infants with and without classical CAH has been known for some ...
متن کاملDimethylmethylene blue-based spectrophotometry of glycosaminoglycans in untreated urine: a rapid screening procedure for mucopolysaccharidoses.
Glycosaminoglycans (GAGs) are measured in urine to screen for mucopolysaccharidoses. Other assay procedures are only qualitative (spot tests), can give false-negative results (spot tests, turbidity tests), or are relatively laborious (uronic acid-carbazole test). The present spectrophotometric procedure, based on the color reaction with dimethylmethylene blue (DMB), can be performed directly on...
متن کاملScreening tests for glycosaminoglycans in urine: experience from regional interlaboratory surveys.
Three urine samples were distributed to laboratories in the Trent and Yorkshire regions to assess their ability to detect glycosaminoglycans. Satisfactory results were obtained for samples from patients with Hunter's and Morquio's diseases but six of 14 laboratories reporting a result for a Sanfilippo sample missed the abnormality. Replies to a subsequent questionnaire showed that unsuccessful ...
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ژورنال
عنوان ژورنال: Archives of Disease in Childhood - Fetal and Neonatal Edition
سال: 2000
ISSN: 1359-2998,1468-2052
DOI: 10.1136/fn.82.1.f77b